- rRNA capture with magnetic beads, so at the end you remove the beads and the sample ends up being mRNA
- degradation of the 5’P RNAs, so mRNAs in bacteria have an analogous to the cap in eukaryotes, a 5’PPP (triphosphate). So the idea is to get rid of all those RNA species, tRNA and rRNA, that does not have a 5’PPP.
- Polyadenylation of mRNA. Here an artificial polyA is added.
- Capture the undesirable RNAs with Hfq, a very famous protein originally discovered in E. coli that binds to RNA.